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单分子力光谱揭示了控制细菌毒素蛋白RTX有效转移的机械设计

Han Wang¹, Xiaoqing Gao², Hongbin Li¹

¹Department of Chemistry , University of British Columbia , Vancouver , British Columbia V6T 1Z1 , Canada.

Journal of the American Chemical Society

|December 3, 2019

概括

细菌腺酸环酶毒素 (CyaA) 的转移受其RTX域的帮助

科学领域:

细菌毒素分泌
蛋白质折叠的动态
单分子生物物理学

背景情况:

腺酸环酶毒素 (CyaA) 需要通过1型分泌系统 (T1SS) 进行有效的转移.
了解CyaA的RTX域的机械特性是阐明转移机制的关键.

研究的目的:

在单个分子水平上研究CyaA的RTX域的机械特性和结构动力学.
阐明RTX域折叠在T1SSCyaA转移中的作用.

主要方法:

使用光学探探测单个RTX域.
分析了Apo和HoloRTX域的机械稳定性和折叠路径.

主要成果:

Apo-RTX域表现为一个随机线圈,方便转移而无阻力.
折叠的全RTX域表现出机械稳定性,并从C端进行向量转换折叠.
通过Ca2+触发的Holo-RTX折叠会产生拉伸力,帮助RTX转移.

结论:

在CyaA转移中,RTX域的Ca2+触发折叠起着至关重要的作用.
提供了控制高效RTX转移的机械设计的机械见解.

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