相关实验视频

Updated: Jan 2, 2026

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

7.4K

细胞中的蛋白质-蛋白质相互作用的光近度分析

David C McCutcheon, Gihoon Lee, Anthony Carlos

    Journal of the American Chemical Society
    |December 11, 2019
    PubMed
    概括

    我们开发了一种新的光近距离蛋白相互作用 (PhotoPPI) 方法来绘制细胞中的蛋白相互作用. 这项技术跟踪动态蛋白质网络,揭示了KEAP1相互作用在细胞压力下如何变化.

    更多相关视频

    Imaging Protein-protein Interactions in vivo11:15

    Imaging Protein-protein Interactions in vivo

    Published on: October 10, 2010

    21.8K
    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces07:42

    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

    Published on: March 19, 2010

    11.1K

    相关概念视频

    Protein-protein Interfaces02:04

    Protein-protein Interfaces

    Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
    14.4K
    Protein Networks02:26

    Protein Networks

    An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
    These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
    4.4K
    Protein Dynamics in Living Cells01:19

    Protein Dynamics in Living Cells

    Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
    Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
    2.6K
    Proteomics01:33

    Proteomics

    A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
    Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
    9.2K