使用Clostridium基因在宿主中的微生物衍生分子的消耗
Chun-Jun Guo1,2, Breanna M Allen3,4, Kamir J Hiam3,4
1Department of Bioengineering and ChEM-H, Stanford University, Stanford, CA 94305, USA.
Science (New York, N.Y.)
|December 14, 2019
概括
研究人员开发了一种修改肠道细菌的方法,特别是在Clostridium Sporogenes中敲除基因. 这表明这些细菌产生的分支短链脂肪酸会调节宿主的免疫球蛋白A活性.
科学领域:
- 微生物学
- 免疫学
- 代谢学
背景情况:
- 肠道微生物组合成了许多宿主循环中的分子.
- 确定这些微生物分子的具体生物学作用是具有挑战性的.
- 克洛斯特里物种是肠道微生物代谢物的重要生产者.
研究的目的:
- 在Clostridium物种中开发针对性基因删除的遗传系统.
- 研究由Clostridium Sporogenes产生的特定分子的宿主调节功能.
- 阐明微生物代谢物在宿主免疫反应中的作用.
主要方法:
- 对Clostridium spp的清洁删除系统的开发
- 该系统应用于模型生物体Clostridium sporogenes.
- 产生一种缺少10 C. 子基因基因的淘汰突变分子.
- 用淘汰突变的小鼠殖民化和宿主免疫参数的分析.
主要成果:
- 在Clostridium spp中成功构建清洁的基因删除.
- 识别分支短链脂肪酸作为主要的C. sporogenes衍生分子.
- 证明这些微生物产品具有免疫球蛋白A调节活性.
- 用淘汰菌株殖民的小鼠显示免疫球蛋白A水平发生变化.
结论:
- 开发的遗传系统使微生物代谢物的功能研究成为可能.
- 由Clostridium sporogenes产生的分支短链脂肪酸直接影响宿主免疫球蛋白A.
- 这项工作提供了对宿主微生物相互作用和肠道细菌免疫调节的见解.
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