通过单细胞基因组学绘制海洋微生物组的复杂性
Maria G Pachiadaki1, Julia M Brown2, Joseph Brown2
1Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, 04544, USA; Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA.
Cell
|December 14, 2019
概括
通过单细胞基因组学恢复了海洋细菌和古生物的基因组. 这项研究揭示了独特的基因组,突出了各种代谢途径,并改善了微生物参考数据库.
科学领域:
- 海洋微生物学
- 基因组学
- 生物地质化学
背景情况:
- 海洋细菌和古生物对全球生物化学循环至关重要.
- 了解复杂的海洋微生物群对于生态和生物技术的进步至关重要.
研究的目的:
- 使用单细胞基因组学探索海洋 prokaryotes 的基因组多样性.
- 确定海洋微生物组中的新代谢途径和生态作用.
- 增强未培养的海洋微生物的基因组数据库.
主要方法:
- 单细胞基因组学被用来恢复部分基因组.
- 使用一种随机的,不依赖假设的细胞选择策略.
- 从0.4毫升的海洋样本中恢复了12715个部分基因组.
主要成果:
- 已知大量的原生浮游生物编码潜力得到恢复,这表明了全球基因组的分散.
- 每个恢复的基因组都是独一无二的, 表明海洋种群的克隆性有限.
- 在多种谱系中发现了许多光采集和二次代谢物生物合成途径.
结论:
- 单细胞基因组学对于发现未培养微生物中的生态作用和生物技术潜力是有价值的.
- 产生的基因组集合显著改善了功能注释和元基因组读数的分类学赋值.
- 这种方法为复杂微生物群落的参考基因组数据库提供了一个模型.
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