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  3. 来自ribonucleotides,arabinonucleotides和2'-deoxynucleotides的可信混合物的rna的出现模型
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  3. 来自ribonucleotides,arabinonucleotides和2'-deoxynucleotides的可信混合物的rna的出现模型

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来自Ribonucleotides,Arabinonucleotides和2'-Deoxynucleotides的可信混合物的RNA的出现模型

Seohyun Chris Kim1,2, Lijun Zhou1,2, Wen Zhang1,2

  • 1Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology , Massachusetts General Hospital , 185 Cambridge Street , Boston , Massachusetts 02114 , United States.

Journal of the American Chemical Society
|January 9, 2020

在PubMed 上查看摘要

概括
此摘要是机器生成的。

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科学领域:

  • 生命研究的起源
  • 前生物化学
  • 分子进化

背景情况:

  • 核糖核酸的非生物合成对于RNA世界假设至关重要.
  • 预生物环境可能产生了核酸的混合物,包括阿拉伯核酸和2'-脱氧核酸.
  • 从复杂的混合物中理解同质RNA的出现是关键.

研究的目的:

  • 调查阿拉伯核酸和2'-脱氧核酸在非酶型模板导向原料扩展中的作用.
  • 确定RNA如何从核酸变异的混合物中出现为主要的遗传聚合物.

主要方法:

  • 使用激活的阿拉比诺核酸和2'-脱氧核酸进行非酶型模板导向的原料延伸反应.
  • 使用 ribo-, arabino- 和 2'-deoxyribonucleotides 的混合物进行实验.
  • 评估初级扩展效率和模板功能.

主要成果:

  • 与核糖核酸相比,非酶原体扩展效率明显低,并强烈抑制进一步扩展.
  • 2'-脱氧核酸也被更低效率地纳入,尽管效果不那么明显.
  • 核酸的混合物没有阻碍RNA模板复制,而嵌合性寡核酸则作为有效的模板.

结论:

  • 模板导向化学可能有利于RNA合成而不是其他核酸变异.
  • 最初的基因聚合物可能是随机的,但复制有利于RNA,导致RNA主导的池.
  • 这为复杂的前生物混合物中出现相对同质的RNA世界提供了一种机制.