B细胞和三级淋巴体结构促进免疫治疗反应
Beth A Helmink1, Sangeetha M Reddy2, Jianjun Gao3
1Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. bhelmink@mdanderson.org.
Nature
|January 17, 2020
概括
不仅仅是T细胞,B细胞对于癌症治疗反应至关重要. 我们的研究表明B细胞特征和三级淋巴体结构预测免疫检查点阻断疗法的成功.
科学领域:
- 免疫学
- 癌症学
- 癌症研究
背景情况:
- 免疫检查点阻断 (ICB) 改变了癌症治疗,但预测生物标志物和反应增强策略主要集中在T细胞上.
- 其他免疫细胞,如B细胞在ICB治疗反应中的作用仍然不太清楚.
研究的目的:
- 研究B细胞和三级淋巴体结构在预测和潜在增强免疫检查点阻塞治疗中的作用.
- 确定ICB治疗疗效的新生物标志物.
主要方法:
- 批量和单细胞RNA测序用于分析ICB治疗患者的瘤样本中的基因表达.
- 计算分析 (MCP计数器) 来评估免疫和层细胞组成.
- 组织学评估和质量细胞测量以检查B细胞局部和表型.
主要成果:
- 在多个ICB队列中,B细胞特异性基因特征在响应者与非响应者的瘤中表达最差异.
- 在三级淋巴体结构中发现了B细胞,反应者显示了B细胞的克隆扩张和独特的功能状态.
- 在响应ICB治疗的患者的瘤中,切换记忆B细胞得到了丰富.
结论:
- B细胞在抗瘤免疫力和对ICB治疗的反应中起着重要作用.
- 三级淋巴体结构与ICB治疗的疗效有关.
- 与B细胞相关的生物标志物和治疗策略有望改善癌症治疗结果.
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