相关实验视频

Updated: May 5, 2026

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

Published on: December 2, 2016

通过光控制的光探针精确监测与二氧化硫水平相关的细胞组织中的热中风

Weijie Zhang¹, Fangjun Huo², Yongkang Yue¹

¹Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science , Shanxi University , Taiyuan 030006 , China.

Journal of the American Chemical Society

|January 18, 2020

概括

研究人员开发了一种新型探测器Ly-NT-SP,用于在热冲击期间检测溶酶体中的二氧化硫 (SO2). 这种进步有助于了解热中风的机制,

科学领域:

生物化学
细胞生物学
化学传感器

背景情况:

热中风对健康构成重大风险,需要对热引起的细胞毒性有更深入的了解.
早期检测和热中风的机理性见解对于开发有效治疗至关重要.

研究的目的:

合成和描述一种新型探测器,Ly-NT-SP,用于检测溶酶体内的二氧化硫 (SO2).
研究SO2在细胞热冲击反应中的作用及其保护作用.

主要方法:

对Ly-NT-SP探针的合成和描述.
探测器的光控制同质化及其与SO2的反应.
使用HeLa细胞的体外选择性和检测研究的极限.
在热冲击期间对溶酶体中的SO2进行活细胞成像.
在细胞和小鼠中进行体内研究以确认SO2的保护作用.

主要成果:

Ly-NT-SP 探针表现出依赖于 pH 的光和光控制的异体化.
光激活的Ly-NT-MR可以选择性地检测SO2的低检测极限 (4.7μM).
该探测器对HeLa细胞无毒,并在热冲击期间成功对溶解体中的SO2进行成像.
在热冲击期间观察到溶酶体中的SO2水平增加.
升高的SO2表明对小肠热冲击引起的损伤有保护作用.

结论:

开发了一种新的,无毒的光探针 (Ly-NT-SP) 用于溶酶体SO2检测.
这项研究提供了第一次实时成像在热冲击期间的溶酶体中的SO2动态.
增加的SO2通过调节氧化应激对热冲击引起的损伤起着保护作用.

更多相关视频

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Published on: June 18, 2020

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...