在毒素体中鉴定分化主调节剂
Benjamin S Waldman1, Dominic Schwarz2, Marc H Wadsworth3
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
Cell
|January 21, 2020
概括
研究人员发现了一个关键的基因开关,BFD1, 控制Toxoplasma gondii的分化. 这一发现为预防和治疗由这种广泛传播的寄生虫引起的慢性感染提供了新的途径.
科学领域:
- 寄生虫学
- 分子生物学
- 遗传学
背景情况:
- 东菌慢性感染全球大约25%的人口.
- 感染的复发会导致免疫力低下的个体出现严重疾病,而其他个体则会出现眼睛问题.
- 治理急性高血压变为慢性变的分子机制在很大程度上是未知的.
研究的目的:
- 阐明Toxoplasma gondii从太基体到白体的分化分子基础.
- 确定控制这一关键发展转变的关键监管者.
主要方法:
- 通过Cas9进行基因查.
- 一个单细胞RNA测序.
- 记者测试以评估基因促进剂的结合.
主要成果:
- 一个类似Myb的转录因子BFD1被确定为差异化必不可少的.
- BFD1积累与引发差异化的压力条件相关.
- 合成表达BFD1足以诱导分化.
- BFD1与特定阶段基因的促进体结合,作为转录调节剂.
结论:
- BFD1作为Toxoplasma gondii分化的一个关键基因开关.
- 了解BFD1的作用为控制寄生虫的发展提供了目标.
- 这一发现对预防和治疗慢性毒素菌感染有意义.
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