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Updated: Dec 30, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

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基因级联放大用于成像RNA亚细胞位置

Kewei Ren1, Rigumula Wu1, Aruni P K K Karunanayake Mudiyanselage1

  • 1Department of Chemistry , University of Massachusetts , Amherst , Massachusetts 01003 , United States.

Journal of the American Chemical Society
|January 23, 2020
PubMed
概括

研究人员开发了一种新的基因编码方法,用于现场活细胞中的RNA成像. 这种技术使用布洛克利RNA记者进行敏感的检测和RNA点的亚细胞定位.

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High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

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Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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