巨型病毒多样性和通过全球转基因组学的宿主相互作用
Frederik Schulz1, Simon Roux2, David Paez-Espino2
1DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. fschulz@lbl.gov.
Nature
|January 23, 2020
概括
研究人员在全球发现了2074个核细胞质大DNA病毒 (NCLDV) 的新基因组. 这大大扩大了我们对病毒多样性及其在生态系统中的作用的理解.
科学领域:
- 病毒学
- 基因组学
- 生态学
背景情况:
- 目前对核细胞质大DNA病毒 (NCLDV) 的理解主要依赖于共同培养的分离物.
- 关于NCLDV的全球多样性和生态作用的知识有限.
研究的目的:
- 从全球基因组数据中重建和分析大量NCLDV基因组.
- 扩大已知的NCLDV的遗传和功能多样性.
- 调查大型和巨型病毒的全球分布和生态意义.
主要方法:
- 使用公开可用的元基因组数据重建2,074个NCLDV基因组.
- 重建基因组的遗传学和功能多样性分析.
- 在元基因组数据集中分析大型和巨型病毒的主要状蛋白.
主要成果:
- 基因多样性增加了11倍,NCLDV的功能多样性扩大了10倍.
- 对大型和巨型病毒的全球分布模式的识别.
- 发现参与光合作用和基质运输的病毒基因,表明宿主重编程策略.
- 横向基因转移的证据将病毒系与多种真核宿主连接起来.
结论:
- 这项研究揭示了以前未知的全球NCLDV的巨大多样性.
- NCLDV,特别是巨型病毒,是全球生态系统的重要参与者,与多种真核细胞系相互作用.
- 转基因学方法是发现病毒多样性和功能的强大工具.
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