在酵母表面上可控制的序列酶的显示,以提高生物催化活性,以实现高效的酶生物燃料细胞
Shuqin Fan1,2, Bo Liang2, Xinxin Xiao1
1Institute for Chemical Biology & Biosensing, and College of Life Sciences , Qingdao University , 308 Ningxia Road , Qingdao 266071 , P. R. China.
Journal of the American Chemical Society
|January 31, 2020
概括
工程酵母细胞显示顺序酶葡萄糖酶 (GA) 和葡萄糖氧化酶 (GOx) 进行增强的生物催化. 这使得酵素生物燃料电池 (EBFC) 能够有效地将粉转化为电力.
科学领域:
- 生物催化和酶工程
- 生物电化学
- 合成生物学
背景情况:
- 酶级联对于多步生物化学反应至关重要.
- 细胞表面显示为酶固定提供了一个强大的平台.
- 生物质转化为电力仍然是酶式生物燃料电池的一个挑战.
研究的目的:
- 在酵母细胞表面构建和优化精确定位的酶级联.
- 研究组装方法对催化活性的影响.
- 开发具有增强性能的新型粉/O2酶生物燃料电池 (EBFC).
主要方法:
- 使用a-聚合素和凝聚素-多克林相互作用,在酵母上同时显示葡萄糖酶 (GA) 和葡萄糖氧化酶 (GOx).
- 优化酶固态度,顺序和分子大小以进行级联组装.
- 用于EBFC的GA/GOx生物电极的组装和表征.
主要成果:
- 与自由酶相比,整体反应速度提高了4倍.
- 最佳的GA/GOx比率 (2:1) 和酶序 (酵母-GA-GOx-GA) 产生了最高的活性.
- 开发了0.78V的开放电路电压和36.1μWcm-2的记录功率密度的EBFC.
结论:
- 序列酶在细胞表面的合理组织显著提高了生物催化活性和稳定性.
- 开发的GA/GOx生物电极是基于粉的EBFC的突破.
- 这种方法对生物催化,生物传感和生物电合成的应用具有前景.
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