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菌体引导向,分辨成像和AIE生物结合物的协同杀死

Xuewen He^1,2, Yujun Yang³, Yongcan Guo⁴

¹Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study, Division of Life Science, Department of Chemical and Biomedical Engineering , The Hong Kong University of Science and Technology , Clear Water Bay , Kowloon Hong Kong.

Journal of the American Chemical Society

|January 31, 2020

概括

研究人员开发了新的生物结合物,将菌体 (PAP) 与聚合诱导排放光原体 (AIEgens) 结合起来. 这些AIE-PAP可通过光动态无活化提供有针对性的细菌成像和有效杀死抗生素敏感和耐药菌株.

科学领域:

生物技术
材料科学
抗菌研究

背景情况:

抗生素耐药性需要新的抗菌策略.
菌体 (PAP) 提供向的细菌感染.
聚合诱导辐射发光剂 (AIEgens) 提供成像和光动力失活 (PDI) 功能.

研究的目的:

创建AIE-PAP生物结合物用于目标细菌成像和协同杀死.
利用菌体的特异性和AIEgen的特性来增强抗菌作用.
开发一种针对抗生素敏感和多药耐药 (MDR) 细菌的新战略.

主要方法:

细菌菌体 (PAP) 与PDI活性AIE基因的结合.
使用菌体向进行特定的细菌识别.
在白光下使用AIEgen光实时跟踪和PDI介导的反应性氧物种 (ROS) 生成.
在体外和体内抗菌疗效测试.

主要成果:

成功生成具有保存菌体向活性的AIE-PAP生物结合物.
通过光成像实时监测生物结合物-细菌相互作用.
通过AIEgens有效生成ROS用于协同杀死细菌.
已证明选择性和协同杀死抗生素敏感和MDR细菌.
在抗菌试验中观察到优异的生物相容性.

结论:

新型AIE-PAP生物结合剂提供了针对性成像和协同杀菌的双重功能平台.
这一综合策略通过针对敏感细菌和MDR细菌有效打击抗生素耐药性.
开发的方法提供了有前途的抗菌剂多样化,并激发了未来的药物开发.

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