植物细胞极性复合体的DIX域聚合驱动器组装
Maritza van Dop1, Marc Fiedler2, Sumanth Mutte1
1Laboratory of Biochemistry, Wageningen University, Stippeneng 4, Wageningen, the Netherlands.
Cell
|February 1, 2020
概括
植物和动物细胞的极性机制有着古老的起源. 植物中的SOSEKI蛋白利用DIX域聚合,这是一个跨王国保存的过程,用于建立细胞极性.
科学领域:
- 细胞生物学
- 发育生物学
- 进化生物学
背景情况:
- 细胞极性对于多细胞生物的发展至关重要.
- 动物平面极性依赖于Wnt信号和Dvl蛋白质聚合.
- 植物的极性机制在很大程度上是未知的.
研究的目的:
- 研究植物和动物细胞极性机制的进化起源.
- 要确定植物极性蛋白是否使用与动物对应物相似的机制.
- 确定调节细胞极性的古老保存途径.
主要方法:
- 在陆地植物中识别和描述SOSEKI蛋白质.
- 分析SOSEKI蛋白质的局部化和聚合.
- 在动物和植物DIX域之间进行跨王国域交换实验.
- 对DIX域进行基因分析.
主要成果:
- 索塞基蛋白是陆地植物中保存的古老的极地蛋白.
- 通过其DIX域,SOSEKI蛋白质聚合,将其他蛋白质招募到极点.
- 动物和植物DIX领域在功能上是相当的,证明了跨王国的保护.
- DIX域可以追溯到单细胞真核生物,早于多细胞性.
结论:
- DIX域依赖的聚合机制是建立细胞极性的一种古老的保存系统.
- 这种机制早于植物和动物多细胞的独立进化.
- 索斯基蛋白质代表了一种被保存的植物对象.
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