从N2转化为H2/α-基托酸酶燃料电池中的生物电催化转化
Hui Chen1, Matthew B Prater1, Rong Cai1
1Department of Chemistry , University of Utah , 315 South 1400 East, Room 2020 , Salt Lake City , Utah 84112 , United States.
Journal of the American Chemical Society
|February 5, 2020
概括
这项研究开发了一种/α-酸酶燃料电池 (EFC),可将气转化为奇拉性氨基酸. 这种自动供电系统提供了一种节能生产有价值的生物化学品的方法.
科学领域:
- 生物技术
- 生物催化
- 绿色化学
背景情况:
- 酶电合成提供了使用电能进行化学生产的可持续途径.
- 酶燃料电池 (EFC) 将化学能量转化为电能,从而实现自动供电反应.
- 将电合成与EFC相结合,可实现高价值化学品的高效,自动生产.
研究的目的:
- 开发一种/α-酸EFC,用于将惰性气转化为性氨基酸.
- 创建一个自动供电的系统从氨和2-基托赫萨诺酸生产l-norleucine.
- 通过生物催化剂来证明一种高能效的替代方法来生产性氨基酸.
主要方法:
- 设计并建造了一种含有酶和酶的阴极反应级联,用于生产氨和NADH.
- 使用氨酸脱酶 (LeuDH) 在现场合成l-norleucine.
- 整合了燃料电池与超热稳定酶 (SHI) 阳极和阴极级联,形成H2/α-酸EFC.
主要成果:
- 在阴极级联中实现了92%的氨转化和87.1%的法拉第效率.
- 在10小时后,H2/α-酸EFC产生了0.36mM的l-norleucine与99%的反体过量.
- 在集成的EFC系统中达到82%的Faradaic效率.
结论:
- 开发的H2/α-酸EFC是一种可行的,节能的生产奇拉氨基酸的方法.
- 该系统利用了 LeuDH 的广泛基质范围和高反选择性,用于生物技术应用.
- 这种方法为传统的电化学方法提供了可持续的替代方案.
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