在活体动物中对原生造血干细胞和原生细胞进行成像
Constantina Christodoulou1,2,3, Joel A Spencer4,5,6,7, Shu-Chi A Yeh4,5
1Stem Cell Program, Boston Children's Hospital, Boston, MA, USA.
Nature
|February 7, 2020
概括
研究人员对活小鼠的静止长期造血干细胞 (HSC) 进行了可视化研究. 他们发现了与骨重塑相关的HSC行为和利基异质性,
科学领域:
- 血液学
- 干细胞生物学
- 微环境动力学
背景情况:
- 研究血造干细胞 (HSC) 生物学传统上依赖于移植模型.
- 在活体动物中可视化其本地骨髓中的HSC行为是一个重大挑战.
研究的目的:
- 开发和应用一种新的内存成像技术,以可视化静止的长期HSCs (LT-HSCs) 在它们的本地位.
- 研究LT-HSC与骨髓微环境相关的空间组织,运动和克隆扩张.
主要方法:
- 在小鼠中采用双重遗传标记策略,专门识别静止的LT-HSC.
- 在活体动物的骨髓内内成像.
- 对血管,内骨表面和缺氧梯度的LT-HSC局部化分析.
- 在稳定状态和激活期间追踪LT-HSC运动和膨胀动态的时间间隔成像.
主要成果:
- 在鼻状血管和内骨表面附近可视化静止的LT-HSCs.
- 在低毒性中发现了LT-HSCs,类似于多能原生细胞 (MPP),但不在最深的低毒性区域.
- 稳定状态的LT-HSCs表现出有限的运动性,而激活的LT-HSCs表现出异质的反应,包括增加的运动性和克隆扩张.
- 主要在骨髓腔中进行活跃骨改造时观察到HSC扩张.
结论:
- 这项研究提供了第一个直接可视化静止的LT-HSC行为在他们的本地利基.
- 骨周转和相关的微环境异质性显著影响HSC的行为和扩张.
- 开发的成像方法允许详细研究HSC和它们在干细胞调节中的作用.
更多相关视频
17:08In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
Published on: August 6, 2014
13.5K
12:54In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Published on: September 4, 2014
14.3K
相关概念视频
00:58Embryonic Stem Cells
25.8K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
25.8K
01:21Hematopoiesis
8.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
8.1K
01:19Multipotency of Hematopoietic Stem Cells
3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
00:57Embryonic Stem Cells
4.5K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.5K
01:20Overview of Hematopoiesis
9.8K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
9.8K