来自地球各个生态系统的巨大菌体
Basem Al-Shayeb1, Rohan Sachdeva1, Lin-Xing Chen1
1Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.
Nature
|February 14, 2020
概括
科学家发现了数百种具有超过200千基基因组的巨型菌体, 这些巨大的菌体拥有广泛的遗传资料, 遍布全球多样化的生态系统.
科学领域:
- 微生物学
- 病毒学
- 基因组学
背景情况:
- 菌体 (菌体) 是感染细菌的病毒,通常具有小的基因组.
- 菌体的复制取决于它们的细菌宿主.
研究的目的:
- 测序和描述来自不同生态系统的大型菌体基因组.
- 研究这些新发现的巨型菌体的遗传和生物策略.
主要方法:
- 从各种环境样本进行DNA测序.
- 完成了35个大型菌体基因组的手动修复.
- 对巨型菌体的主要分类进行基因分析.
主要成果:
- 发现了超过200千基的数百个菌体基因组,包括迄今为止最大的735千基基因组.
- 扩展基因库的识别,包括新型CRISPR-Cas系统,tRNA和蛋白质合成机制.
- 菌体利用CRISPR-Cas系统来操纵宿主基因表达并消除竞争性菌体.
- 巨大的菌体在遗传学上被定义并分布在不同的环境和宿主范围.
结论:
- 在巨大的菌体中大量的基因库存代表了一种保存的生物策略.
- 这些菌体分布在全球,感染广泛的细菌宿主.
- 这一发现扩大了我们对菌体多样性,进化和生态作用的理解.
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