哺乳动物中枢神经系统中发育的轴突的化学导向指导
M Tessier-Lavigne1, M Placzek, A G Lumsden
1Center for Neurobiology and Behavior, Columbia University, New York 10032.
Nature
|December 22, 1988
概括
中枢神经系统中发展的轴突是由化学吸引分子指导的. 床板细胞分泌的因素指导 commissural 轴突的生长,支持脊椎动物中枢神经系统的化学导向.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 轴突指导对神经系统发育至关重要,依赖于细胞表面和细胞外矩阵线索.
- 在脊椎动物的中枢神经系统 (CNS) 中,建议使用分子梯度引导的化学化,但尚未得到证实.
- 脊髓中的 commissural 轴突导航到地板板,一个中线结构.
研究的目的:
- 为了研究化学转移机制是否引导脊椎动物中枢神经系统中发育的轴突.
- 为了确定地板细胞是否分泌影响轴突生长模式的扩散性因素.
- 为了测试化学导向对 commissural 轴突的假设.
主要方法:
- 在实验室共培养试验中,使用大鼠地板细胞和胚胎脊髓细胞.
- 分析地板细胞分泌物对 commissural轴突生长模式和方向的影响.
- 评估不同胚胎脊髓轴突上的指导线索的特异性.
主要成果:
- 鼠床板细胞分泌扩散性因子,这些因子在体外吸引和定向 commissural 轴突.
- 这些因素特别影响了指导性轴突的生长,而不会影响其他胚胎脊髓轴突.
- 观察到的效应支持一种化学导向机制.
结论:
- 化学化在引导发育中的轴突到脊椎动物中枢神经系统中的目标中起着重要作用.
- 由地板细胞分泌的扩散性化学吸引剂是指导性轴突指导的关键媒介.
- 这项研究提供了中枢神经系统中化学药物引导的证据.
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