人类肺部的DNA adduct水平与吸烟的相关性
D H Phillips1, A Hewer, C N Martin
1Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Nature
|December 22, 1988
概括
吸烟显著增加了肺部的DNA附加物,这是癌症风险的关键指标. 水平与吸烟量相关,在五年无烟后恢复正常.
科学领域:
- 在瘤学瘤学.
- 环境健康 环境健康
- 分子生物学分子生物学
背景情况:
- 肺癌是癌症死亡的主要原因,与吸烟有很强的联系.
- 香烟烟雾中含有众多致癌物质,但与肺部DNA损伤的直接联系尚未得到充分研究.
- DNA附加物是致癌物暴露的生物标志物,但它们在人类肺组织中的水平尚未得到充分记录.
研究的目的:
- 从吸烟者和非吸烟者的人类肺组织中量化DNA adduct水平.
- 为了研究吸烟消费和肺部DNA添加水平之间的关系.
- 为了评估戒烟后DNA附带形成的可逆性.
主要方法:
- 利用32P后标记技术进行敏感的DNA adduct检测.
- 分析了不同吸烟史的个体的肺组织样本.
- 与日常和终身吸烟消费相关的DNA adduct水平.
主要成果:
- 与非吸烟者相比,吸烟者在肺组织中显示出显著更高的DNA添加水平.
- 在吸烟量 (每日和终身) 和吸烟水平之间观察到线性关系.
- 戒烟五年或更长时间的个体显示DNA添加水平与非吸烟者相似.
结论:
- 吸烟与人类肺组织中DNA adduct形成的增加直接相关.
- 吸烟导致的肺损伤的定量生物标志物是DNA补充水平.
- 戒烟有效地减少了这些DNA添加物,减轻了长期的癌症风险.
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