转录因子AP2控制了类生殖细胞的诱导
Timothy Q DuBuc1, Christine E Schnitzler2,3, Eleni Chrysostomou1
1Centre for Chromosome Biology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Science (New York, N.Y.)
|February 15, 2020
概括
转录因子AP2 (Tfap2) 将成年干细胞与克隆动物的生殖细胞结合起来. 这一发现揭示了各种动物群体中生殖细胞发育的保存机制.
科学领域:
- 发育生物学
- 干细胞生物学
- 进化生物学
背景情况:
- 克隆动物缺乏隔离的生殖系,依赖于成年干细胞进行配体生产.
- 在克隆动物中诱导生殖细胞命运的机制在很大程度上是未知的.
- 在非隔离动物中理解生殖细胞的发展可以阐明保存的进化途径.
研究的目的:
- 调查转录因子AP2 (Tfap2) 在克隆性鱼*Hydractinia symbiolongicarpus*中的生殖细胞参与.
- 确定Tfap2是否对该物种的生殖细胞和性腺发育至关重要.
- 为了比较克隆和双边动物之间的生殖细胞诱导机制.
主要方法:
- 在Hydractinia symbiolongicarpus中使用了*Tfap2*基因淘汰突变.
- 使用野生类型和突变细胞进行细胞移植实验.
- 研究了强制Tfap2表达在成年干细胞 (i细胞) 的作用.
主要成果:
- *Tfap2*突变体完全没有生殖细胞和生殖腺.
- 移植的野生细胞可以恢复性腺发育,但不能在突变者中诱导生殖细胞.
- 在i细胞中强制表达Tfap2成功诱导了生殖细胞命运.
结论:
- 转录因子AP2 (Tfap2) 是Hydractinia symbiolongicarpus*中生殖细胞结合的关键调节剂.
- Tfap2特别对成年干细胞起作用,诱导生殖细胞的命运,独立于性腺形成.
- Tfap2在生殖细胞结合中的作用在有或没有隔离生殖细胞的动物中保持不变.
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