主体-病原体相互作用的泛基因组效应器触发免疫场景
Bradley Laflamme1, Marcus M Dillon1, Alexandre Martel1
1Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada.
植物免疫力依赖于对病原体的效应激发免疫力 (ETI). 这项研究揭示了Arabidopsis thaliana对Pseudomonas syringae效应体的广泛ETI识别,确定了新的免疫受体,并突出了广泛的病原体覆盖范围.
科学领域:
- 植物病理学
- 分子植物微生物相互作用
- 基因组学
背景情况:
- 效应器触发免疫 (ETI) 对于植物对病原体的防御至关重要.
- 缺乏对不同病原体群体中外传染性感染的全面了解.
- 假是一种具有广泛效应因子多样性的重要植物病原体.
研究的目的:
- 系统地调查针对广泛的Pseudomonas注射器效应物的ETI流行情况.
- 识别参与识别病原体的新型植物免疫受体.
- 评估Arabidopsis thaliana对广泛疾病的抗性潜力.
主要方法:
- 由5127个独特的效应蛋白构建的Pseudomonas注射器类型III效应组合 (PsyTEC).
- 在模型植物Arabidopsis thaliana上对PsyTEC进行查,以识别引起ETI的等位基因.
- 新宿主免疫受体的识别和表征,包括CAR1和ZAR1.
主要成果:
- 在Arabidopsis thaliana中发现了来自19个效应体家族的59个ETI诱导基因 (11.2%)
- 这些引发ETI的等位基因保留,在96.8%的Pseudomonas syringae菌株中发现了正基因基因.
- 确定了两种新的宿主免疫受体,即CAR1和ZAR1,其中CAR1识别了AvrE和HopAA1的保存效应体.
- 一个高百分比的Pseudomonas syringae菌株 (94.7%) 拥有由CAR1或ZAR1识别的等位基因.
结论:
- 对于Pseudomonas syringae的有效剂多样性,ETI提供了广泛的耐药性.
- 鉴定CAR1和ZAR1,并承认它们是保存的效应因子,为植物的抗病工程提供了新的目标.
- 这项工作为开发针对Pseudomonas syringae的持久抵抗策略奠定了基础.
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