Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gastrulation01:56

Gastrulation

65.2K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
65.2K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

6.3K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Reply: Stem cell-based embryo models: scientific promise clashes with ethical reality.

Human reproduction (Oxford, England)·2026
Same author

Retinoic acid and FGF signaling interact to control elongation and lineage specification in a mouse gastruloid model.

Development (Cambridge, England)·2026
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

DNA repair drives cisplatin-induced neuronal death.

Cell·2026
Same author

Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells.

Development (Cambridge, England)·2026
Same author

Stem cell-based embryo models as a tool for reproductive biology.

Molecular human reproduction·2026

相关实验视频

Updated: Dec 28, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.6K

单细胞和空间转录学揭示了胃类动物的体生

Susanne C van den Brink1, Anna Alemany2, Vincent van Batenburg2

  • 1Oncode Institute, Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Utrecht, The Netherlands. s.c.vandenbrink@gmail.com.

Nature
|February 21, 2020
PubMed
概括

胃类细胞,或三维干细胞聚合物,密切模仿哺乳动物的胚胎发育. 这项研究验证了它们在体外生殖和发育过程中的使用.

更多相关视频

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
10:41

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Published on: July 24, 2014

14.2K
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.3K

相关实验视频

Last Updated: Dec 28, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.6K
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
10:41

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Published on: July 24, 2014

14.2K
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
11:52

Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps

Published on: February 9, 2017

6.3K

科学领域:

  • 发育生物学
  • 干细胞生物学
  • 基因组学

背景情况:

  • 来自胚胎干细胞的胃类细胞是早期哺乳动物发育的模型.
  • 与胚胎相比,胃类动物的全基因表达在很大程度上尚未被探索.
  • 了解胃类发育模拟对于它们的应用至关重要.

研究的目的:

  • 综合比较小鼠胃类动物和胚胎之间的基因表达模式.
  • 识别新型细胞类型和胃类动物的发展过程.
  • 建立胃类动物作为发育研究的高通量体外模型.

主要方法:

  • 胃类动物和胚胎的单细胞RNA测序 (scRNA-seq).
  • 空间转录学以绘制3D基因表达.
  • 通过实时成像观察一些动态的发育过程,
  • 信号通道的高通量选.

主要成果:

  • 在胃类动物中发现了以前未知的胚胎细胞类型.
  • 在胃类动物和胚胎之间表现出关键的体生成调节剂的相似表达.
  • 在胃类动物中证实了活体体发生的时钟动态,反映了体内过程.
  • 透露了FGF信号在诱导短尾表型中的作用.
  • 显示的Matrigel嵌入诱导了正确的角形-尾形索米特图案.

结论:

  • 胃类动物准确地回顾了胚胎发育的关键方面,包括体生.
  • 胃素作为一种强大且可扩展的体外模型,用于高通量发育生物学研究.
  • 这项研究提升了我们对早期哺乳动物发育的理解,并为未来的研究提供了坚实的平台.