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精确和可编程的突变检测使用超特异性肋骨调节器

Fan Hong¹, Duo Ma¹, Kaiyue Wu¹

¹Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA; School of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.

|February 29, 2020

概括

科学家开发了单核酸特异性可编程核糖调节器 (SNIPRs),用于超特异性RNA检测. 这些SNIPR可以精确识别活细胞和临床样本中的单核酸突变.

科学领域:

分子生物学
合成生物学
生物技术

背景情况:

识别单核酸突变对于了解细胞生物学和疾病诊断至关重要.
在复杂的生物系统中检测微妙的单基变化存在挑战,

研究的目的:

开发一种用于超特异性RNA检测的新型 prokaryotic riboregulators.
为了在体内和体外准确识别单核酸突变.

主要方法:

单核酸特异性可编程核糖调节器 (SNIPR) 的新设计.
在大肠杆菌 (E. coli) 中测试SNIPR性能,以检测基因表达的差异.
在无细胞转录翻译系统中使用SNIPR进行体外分析.
为设计针对特定突变的SNIPR开发自动化算法.
将SNIPR与基于纸张的无细胞反应集成为同热检测.

主要成果:

在对大肠杆菌单核酸变异的反应中,SNIPR实现了超过100倍的基因表达差异.
在实验室中,SNIPRs 显示出单个表皮转录标记的解脱能力.
针对与癌症,耐药性和遗传性疾病相关的突变而设计的SNIPR.
通过色度检测能够方便地对癌症突变和寨卡病毒菌株进行同热检测.

结论:

新设计的SNIPR为超特异性RNA检测和单核酸突变识别提供了强大的工具.
在各种环境中,SNIPR可提供精确的分子诊断和生物探测.
与便携式无细胞系统的整合扩大了先进的分子检测方法的可用性.

关键词:

颜色测量在诊断同热的发生变异基于纸质的测试体调节器特定性

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