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酵母酵母中的多基化RNA序列的数量和分布

L M Hereford, M Rosbash

|March 1, 1977

概括

研究人员使用RNA-cDNA杂交方法分析了酵母中的多基尼化RNA. 他们发现酵母有3000-4000个不同的信使RNA序列,其中大多数存在于多体体上.

科学领域:

分子生物学分子生物学
酵母遗传学酵母遗传学
单核生物基因表达的表达方式

背景情况:

聚氨基化RNA在真核生物基因调节中起着至关重要的作用.
之前的研究表明,甲基动物真核生物中的RNA复杂性.
了解酵母RNA复杂性,可以了解基本的细胞过程.

研究的目的:

确定来自Saccharomyces cerevisiae的poly(A) 含RNA中的独特序列的数量和分布.
为了比较酵母中的RNA复杂性与甲基动物真核生物的复杂性.
为复杂性分析评估RNA-cDNA杂交的准确性.

主要方法:

对RNA-cDNA杂交的动态分析.
用单拷贝DNA进行杂交,以验证序列计数.
含有多元A的多体RNA与总RNA的比较.

主要成果:

酵母中含有多A的RNA具有不同的复杂度等级:低 (~20),中 (~400) 和高 (~2400) 序列.
大约20%的单拷贝酵母基因组是由多基化RNA所代表,相当于约4000个序列.
大多数序列复杂性都在多聚体上发现,并且是腺化,在总RNA中分布相似.

结论:

RNA-cDNA杂交是一种可靠的测量序列复杂性的方法.
植物生长的酵母具有估计3000-4000个不同的信使RNA序列.
这些发现与以前对其他真核生物的RNA复杂性的描述一致.

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