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Updated: Dec 25, 2025

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没有蛋白质的表达蛋白质结合

Yuchen Qiao1, Ge Yu1, Kaci C Kratch1

  • 1The Texas A&M Drug Discovery Laboratory, Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States.

Journal of the American Chemical Society
|March 28, 2020
PubMed
概括

这项研究引入了激活的囊导向蛋白质结合 (ACPL),一种新型的无酶蛋白质合成方法. ACPL通过化学激活氨酸残留物,使其能够有效地结合蛋白质,用于各种应用.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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