静电相互作用控制细菌冰核的功能
M Lukas1, R Schwidetzky1, A T Kunert2
1Max Planck Institute for Polymer Research, 55128 Mainz, Germany.
Journal of the American Chemical Society
|April 1, 2020
概括
细菌的冰核蛋白 (INP) 通过静电相互作用聚合,提高它们的冰核效率. 这种聚合取决于pH值,对于压力下的细菌冰形成至关重要.
科学领域:
- 微生物学
- 生物物理
- 材料科学
背景情况:
- 细菌的冰核蛋白 (INPs) 在促进冰的形成方面非常有效.
- 它们的高活性与功能性INP聚合物的形成有关.
- 人们对INP活动的确切机制尚不完全了解.
研究的目的:
- 调查静电相互作用在Pseudomonas注射器中的INPs冰核活动中的作用.
- 将INP的电荷状态与它们的冰核效率相关联.
- 阐明激活INP聚合物的形成背后的机制.
主要方法:
- 使用高通量冰核测试.
- 使用特定表面的总频率生成光谱.
- 在一系列的pH值中确定非活性的P.注射器的充电状态.
主要成果:
- INP聚合物的冰核活性与净电荷有很强的相关性,在同电点上显示最小的活性.
- 与聚合物相比,INP单体活性对pH变化不那么敏感.
- 水分子的界面对齐,表明电荷状态,直接与核化活动有关.
结论:
- 静电相互作用对于形成高活性,功能一致的INP聚合物至关重要.
- 这种机制解释了细菌在压力条件下如何促进冰核形成.
- 这些发现提供了细菌冰核形成的分子基础.
更多相关视频
08:39Electroporation of Functional Bacterial Effectors into Mammalian Cells
Published on: January 19, 2015
10.4K
09:54Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
Published on: November 19, 2015
11.1K
相关概念视频
Intracellular Movement of Viruses and Bacteria
3.3K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.3K
Colloidal precipitates
4.5K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
4.5K
Other Unique Bacteria
322
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
322
Noncovalent Attractions in Biomolecules
62.8K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
62.8K
Bacterial Signaling
39.8K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
39.8K
Cytoskeletal Proteins in Bacteria
4.0K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
4.0K
