实时光现场 隐藏指纹的可视化 超出3级细节 基于聚合诱导的发射
Ya-Long Wang1, Chong Li1, Hong-Qing Qu1
1Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Journal of the American Chemical Society
|April 1, 2020
概括
一种新型的水溶性探针,TPA-1OH,可以在各种表面上实时,无毒的光可视化隐藏的指纹 (LFP). 这种聚合诱导的排放探测器清楚地揭示了指纹的详细特征,包括3次纳米级信息.
科学领域:
- 法医科学
- 材料科学
- 分析化学
背景情况:
- 隐藏的指纹在法医调查中至关重要.
- 目前的LFP可视化方法通常需要有机溶剂和后处理,限制实时分析.
- 开发用于LFP检测的灵敏和高效的探测器仍然是一个关键挑战.
研究的目的:
- 合成具有聚合诱导排放 (AIE) 特性的水溶性探针,用于隐藏指纹可视化.
- 为了实时展示探测器的有效性,在各种基板上对LFP进行现场光成像.
- 评估开发的探测器可以达到的细节和分辨率.
主要方法:
- 一个水溶性AIE探针的合成,TPA-1OH.
- 制备TPA-1OH水溶液 (30 μM).
- 通过浸泡或喷涂LFP的基板,然后通过405nm光激发探针的应用.
- 在现场进行实时光成像和分析已开发的LFP.
- 对探针的细胞毒性评估.
主要成果:
- TPA-1OH在水中表现出非光,但在聚合时表现出强光.
- 在没有有机共溶剂或后处理的情况下,在各种表面 (墙壁,,纸张) 上成功可视化LFP.
- 在50μM以下的度下,探针的非细胞毒性.
- 实时成像捕捉了发展过程, 显示强度和时间之间的指数关系.
- 开发的LFP图像揭示了1-3级细节,包括脊梁宽度和汗孔特征.
- 超分辨率显微镜可视化超过3级的纳米细节,分辨率低于50nm.
结论:
- TPA-1OH是一种有效的水溶性AIE探针,用于实时隐藏指纹可视化.
- 该探针可以在非细胞毒性条件下对指纹特征进行详细分析,包括纳米信息.
- 这种方法为法医应用提供了有前途的进步,提高了隐藏指纹检测的灵敏度和分辨率.
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