中心体定调节原生物质和皮质形成
Wei Shao1,2, Jiajun Yang3, Ming He3,4
1Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Centre, New York, NY, USA.
Nature
|April 3, 2020
概括
将中心体固定在顶膜上可以控制放射性质细胞 (RGP) 的机械特性. 这调节了RGP的扩散,影响了哺乳动物的大脑皮层的大小和折叠.
科学领域:
- 神经科学
- 发育生物学
- 细胞生物学
背景情况:
- 放射性质前体细胞 (RGP) 在哺乳动物发育大脑皮层中产生神经元和质细胞至关重要.
- RGP 中的中心体的顶端位置是一个独特的亚细胞组织,其分子基础和功能尚不清楚.
研究的目的:
- 在皮层RGP中研究中心体对膜的分子机制和功能意义.
- 阐明中心体组织在调节RGP行为和大脑皮层发育中的作用.
主要方法:
- 使用小鼠模型来研究中心体蛋白83 (CEP83) 的功能.
- 研究了CEP83去除对中心体定,细胞力学和RGP增殖的影响.
- 评估了YAP信号通路的参与,以应对改变的中心体组织.
主要成果:
- 在RGP中,CEP83对于形成将母心与顶膜固定的远端附属物至关重要.
- 移除CEP83会破坏中心体的定,导致微管的失调和顶膜机制的改变.
- CEP83的枯竭激活了YAP信号,导致过度的RGP增殖,增加了中间祖细胞,以及扩大,异常折叠的皮质.
- 同时消除YAP可以挽救CEP83移除所引起的皮质扩大和折叠缺陷.
结论:
- 对于调节皮质RGP的机械性质而言,中心体对顶膜的定至关重要.
- 这种机械调节会影响RGP线粒细胞的行为,前代细胞的产生,最终影响哺乳动物大脑皮层的大小和复杂的折叠.
- 揭示了中心体在控制神经前代细胞行为和大脑发育的机制传导途径中的新角色.
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