早期的命运定义了微细胞和非细胞的大脑细胞的发展
Sebastian G Utz1, Peter See2, Wiebke Mildenberger1
1Institute of Experimental Immunology, University of Zurich, 8057 Zurich, Switzerland.
Cell
|April 8, 2020
概括
中枢神经系统 (CNS) 巨细胞,包括微细胞和边界相关巨细胞 (BAMs),起源于不同的发育途径. 这项研究揭示了它们的单独本体和独特的TGF-β依赖性.
科学领域:
- 神经免疫学
- 发育生物学
- 细胞和分子神经科学
背景情况:
- 中枢神经系统 (CNS) 巨由脑膜和边缘相关巨 (BAMs) 组成,这些巨位于脑膜,胸膜和周围血管空间.
- 虽然大多数中枢神经系统的巨细胞是在胚胎发生过程中形成的,但状和长巨细胞是成年单细胞衍生的,这引发了关于微细胞和BAM发育起源的问题.
- 微质和BAM的共同或不同的发育程序在很大程度上是未知的.
研究的目的:
- 研究中枢神经系统的不同巨细胞群体,特别是微质细胞和BAM的本体生长和发育要求.
- 确定微质和BAM是否来自单独的血统,并确定它们的祖先来源.
- 阐明TGF-β在这些巨细胞群的发展中的作用.
主要方法:
- 在发育过程中根据表型,转录和空间特征识别不同的大脑巨细胞群.
- 使用命运映射模型追踪微细胞和BAMs的发育起源.
- 研究微质和BAM发育对TGF-β信号的依赖性.
主要成果:
- 两种不同的大脑巨细胞群,是小质细胞和BAM的前体,在发育早期被发现,在黄囊中已经存在分离的证据.
- 结果显示,BAMs主要来自黄囊中的早期红骨髓前代.
- 发现微细胞的发育依赖于TGF-β,而BAM的产生则独立于该细胞因子.
结论:
- 发育中的对细胞和非对细胞的大脑巨细胞代表着具有不同本体遗传起源的单独实体.
- 这些巨细胞群体在发育过程中表现出独特的基因特征和TGF-β的差异性需求.
- 这些发现澄清了关键中枢神经系统巨群体的单独发育轨迹.
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