通过彻底的基因控制, 揭示了呼吸道保护方案
Sara L Prescott1, Benjamin D Umans1, Erika K Williams1
1Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|April 8, 2020
概括
研究人员发现罕见的喉部感觉神经元保护呼吸道. 激活这些P2RY1神经元会触发呼吸道防御反应, 这对于防止吸血和窒息至关重要.
科学领域:
- 神经科学
- 感官生物学
- 神经功能
背景情况:
- 喉感应神经元功能障碍会导致危及生命的情况,
- 喉防御反射所涉及的特定感官通路尚未得到充分理解.
研究的目的:
- 鉴定和描述喉神经元,保护呼吸道的罕见感觉神经元.
- 阐明这些神经元在启动协调的呼吸道防御机制中的作用.
主要方法:
- 用于绘制,切除和光遗传控制的迷你和光喉感官神经元的综合遗传图谱.
- 使用光遗传学研究神经元反应,并评估针对性神经元切除后的保护性反射.
主要成果:
- 鉴定出每只小鼠大约100个喉内P2RY1神经元的罕见群体.
- 性P2RY1神经元的光遗传激活引发了一种协调的呼吸道防御程序,包括呼吸暂停,声引进,吞和呼气反射.
- 这些P2RY1神经元的消去消除了对喉水和酸的保护性反应.
- 解剖测绘显示P2RY1神经元形成与喉味蕾相邻的体质末端,从上皮细胞接收ATP信号.
结论:
- 性P2RY1神经元是保护呼吸道的关键哨兵, 启动必要的防御反应.
- 皮质细胞通过ATP与P2RY1神经元进行通信,突出显示一种新的感觉通路.
- 这些发现为呼吸道防御提供了机械的洞察力,并为理解内脏器官中迷走神经介导的感觉提供了路线图.
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