相关实验视频
Updated: Dec 22, 2025
15:49
Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
Published on: June 9, 2022
1.8K
使用合成基因组学平台快速重建SARS-CoV-2
Tran Thi Nhu Thao1,2,3, Fabien Labroussaa2,4, Nadine Ebert1,2
1Institute of Virology and Immunology (IVI), Bern, Switzerland.
Nature
|May 5, 2020
概括
一种基于酵母的新型合成基因组平台能够快速重建各种RNA病毒,包括SARS-CoV-2. 这种强大的逆转基因系统加速病毒研究和应对新出现的传染病.
科学领域:
- 病毒学
- 合成生物学
- 分子生物学
背景情况:
- 大型RNA病毒基因组对大肠杆菌的传统克隆和操纵构成挑战.
- 现有的逆转基因系统可能是繁的,阻碍了对病毒爆发的快速研究和应对.
研究的目的:
- 开发和验证基于酵母的快速和强大的合成基因组平台,用于重建多种RNA病毒.
- 为了使RNA病毒,包括冠状病毒的有效基因操纵和表征.
主要方法:
- 使用Saccharomyces cerevisiae进行病毒亚基因组片段的一步重组,使用转换相关的重组克隆.
- 将病毒基因组生成为酵母的人造染色体.
- 使用T7RNA聚合酶产生传染性RNA用于病毒救援.
主要成果:
- 成功重建了来自Coronaviridae,Flaviviridae和Pneumoviridae家族的各种RNA病毒.
- 在接收合成DNA后的一周内,设计和生成化学合成的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) 克隆.
- 展示了该平台的实时生成和演变RNA病毒变异的功能特征的能力.
结论:
- 基于酵母的合成基因组平台为RNA病毒逆转基因提供了强大而有效的工具.
- 这项技术有助于快速应对新出现的病毒威胁,有助于致病研究和疫苗开发.
- 该平台支持合成病毒克隆的生成和分析,对于了解和对抗COVID-19等病毒性疾病至关重要.
更多相关视频
08:41Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
Published on: November 5, 2021
3.2K
12:20Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
Published on: December 29, 2015
21.8K
相关概念视频
02:55Synthetic Biology
5.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
5.4K
02:35RACE - Rapid Amplification of cDNA Ends
7.0K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.0K