基因诱导的BIK1单化调节植物免疫力
Xiyu Ma1,2, Lucas A N Claus3,4, Michelle E Leslie5,6
1Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.
Nature
|May 15, 2020
概括
植物免疫受体检测病原体的模式, 激活防御. 这项研究揭示了BOTRYTIS诱导的KINASE 1 (BIK1) 是单一的,它从FLAGELLIN SENSING 2 (FLS2) - BRASSINOSTEROID INSENSITIVE 1- ASSOCIATED KINASE 1 (BAK1) 复合物中释放出来,从而触发免疫力.
科学领域:
- 植物免疫力
- 分子信号
- 细胞过程
背景情况:
- 植物免疫受体 (PRR) 识别了与微生物相关的分子模式 (MAMPs),以启动防御反应.
- 类似受体的细胞质激酶 (RLCK) 是与PRR相关的关键调节剂,但它们的激活机制在很大程度上尚不清楚.
- 炎诱导的KINASE 1 (BIK1) 是一个关键的RLCK参与植物免疫信号.
研究的目的:
- 阐明植物免疫中RLCK激活的分子机制.
- 研究蛋白质酸化和无化在调节PRR-RLCK复合体动态中的作用.
- 了解BIK1是如何从FLAGELLIN SENSING 2 (FLS2) - BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) 综合体中激活和释放的.
主要方法:
- 在使用先进的显微镜技术检测MAMP后研究了BIK1的动态.
- 使用遗传方法来识别涉及BIK1修饰的E3无素连接酶.
- 分析了BIK1对联体结合的酸化和单化状态.
主要成果:
- MAMP的感知触发了BIK1的酸化,随后是单化.
- 阿拉比多普西斯E3泛酶RING-H2 FINGER A3A (RHA3A) 和RHA3B介导了BIK1单泛.
- 在FLS2- BAK1复合体中释放BIK1,并随后激活免疫信号的过程中,单双化是必不可少的.
- BIK1的动态,包括单体化和内体局部化,与PRR FLS2的动态不同.
结论:
- 联体诱导的单双化是控制RLCK激活和PRR复合体释放的关键调节机制.
- 化和无化之间的相互作用决定了PRR-RLCK复合体的激活和植物免疫反应.
- 这项研究揭示了通过RLCK的动态调节激活植物免疫信号的新途径.
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