通过人类单克隆SARS-CoV抗体对SARS-CoV-2进行交叉中和
Dora Pinto1, Young-Jun Park2, Martina Beltramello1
1Humabs BioMed SA, Vir Biotechnology, Bellinzona, Switzerland.
Nature
|May 19, 2020
概括
在一名SARS-CoV幸存者身上发现了一种强大的中和抗体S309. 这种抗体有效地向SARS-CoV-2尖端糖蛋白,为COVID-19 (冠状病毒疾病2019) 的治疗和预防提供了潜在的可能性.
科学领域:
- 病毒学
- 免疫学
- 结构生物学
背景情况:
- 由SARS-CoV-2引起的持续的COVID-19大流行需要紧急开发疫苗和治疗方法.
- 由于其在宿主细胞进入中的作用,SARS-CoV-2尖端 (S) 糖蛋白是中和抗体的关键目标.
- 以往与SARS-CoV (严重急性呼吸道综合征冠状病毒) 的经验为了解交叉反应性免疫提供了基础.
研究的目的:
- 识别和描述针对SARS-CoV-2的强效中和单克隆抗体.
- 研究有前途的抗体候选者的作用机制和表位识别.
- 探索抗体尾酒的潜力,
主要方法:
- 从有SARS-CoV感染史的个体中分离出记忆B细胞.
- 针对SARS-CoV-2尖端糖蛋白的单克隆抗体的查和特征.
- 伪病毒和真实的病毒中和试验.
- 低温电子显微镜和结合测试以确定表位和与受体结合域的相互作用.
主要成果:
- 鉴定了S309,一种对SARS-CoV-2和SARS-CoV有效的强效中和抗体.
- S309与S糖蛋白的受体结合域结合,识别了Sarbecovirus亚系中的一个保存的糖表位.
- S309与宿主细胞受体的附着不竞争.
- 包括S309在内的抗体合剂表现出增强的中和和限制病毒逃逸的潜力.
结论:
- S309是一种有前途的COVID-19治疗候选物,针对SARS-CoV-2尖端蛋白的保存表位.
- 抗体尾酒提供了一种提高中和效率和克服病毒抗性的策略.
- 这些发现支持开发基于S309的预防和治疗SARS-CoV-2感染的疗法.
更多相关视频
07:08A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
Published on: April 6, 2021
5.4K
08:07Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
Published on: January 12, 2024
1.1K
相关概念视频
00:42Cross-reactivity
32.7K
Overview
32.7K
01:31Hybridoma Technology
16.9K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
16.9K
01:26Antibody Actions
2.2K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
2.2K