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通过自动流化学合成蛋白质

N Hartrampf¹, A Saebi¹, M Poskus¹

¹Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Science (New York, N.Y.)

|May 30, 2020

概括

自动流动化学可以快速直接制造长达164个氨基酸的蛋白质. 这种没有核糖体的方法可以产生与细胞相似的合成蛋白质.

科学领域:

生物化学
化学工程
合成生物学

背景情况:

核糖体是蛋白质合成的细胞机械,仅限于20个标准氨基酸.
目前的蛋白质生产方法通常依赖于生物系统.

研究的目的:

开发和展示一种用于蛋白质生产的自动化,快速化学合成方法.
为制造功能性蛋白质创造一种无核糖体的替代品.

主要方法:

使用高效率的自动化快速流量仪器直接制造链.
进行了超过327次连续反应,合成高达164个氨基酸的链.
合成了九种不同的蛋白质链,包括酶和调节因子.

主要成果:

实现快速链延长,在几个小时内完成合成.
证明了蛋白质生产的高可靠性自动化流体化学.
纯化和折叠的合成蛋白质表现出与生物表达蛋白质相比的生物物理和酶特性.

结论:

自动流体化学为核糖体蛋白合成提供了可行的,快速的替代方案.
这种方法允许直接化学制造功能单域蛋白.
这项技术超越了生物蛋白质生产系统的局限性.

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