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Updated: Dec 19, 2025

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

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对"使用CRISPR相关转基因酶进行RNA导向DNA插入"的评论的回应

Jonathan Strecker1,2,3,4, Alim Ladha1,2,3,4, Kira S Makarova5

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|June 6, 2020
PubMed
概括

与CRISPR相关的转基因ShCAST系统可以在大肠杆菌中整合供体质粒,但使用5'切断的DNA供体可以防止这种不良结果. 这说明了ShCAST系统在基因工程中的应用.

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