相关实验视频
Updated: Jun 9, 2026
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Published on: May 24, 2017
全球RNA-RNA空间相互作用的现场分析
Zhaokui Cai1,2, Changchang Cao1, Lei Ji1
1Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Nature
|June 6, 2020
概括
研究人员开发了RNA在位构成测序 (RIC-seq) 来绘制细胞内的RNA结构和相互作用. 这项新技术揭示了全球RNA相互作用,识别了调节元素,并揭示了基因调节的机制.
科学领域:
- 分子生物学
- 基因组学
- 生物化学
背景情况:
- RNA分子对于生物过程至关重要,经常形成复杂的结构并与蛋白质相互作用.
- 了解完整细胞内的RNA结构和相互作用是必不可少的,但在技术上具有挑战性.
- 目前的方法缺乏在原生细胞环境中对RNA相互作用进行全球分析的分辨率.
研究的目的:
- 开发一种在现场进行RNA-RNA相互作用的综合分析的新技术.
- 在人体细胞内生成RNA的三维 (3D) 交互图.
- 探索RNA相互作用在基因表达和细胞组织中的调节作用.
主要方法:
- 在位配列RNA (RIC-seq) 配对近距离结合与深度配列.
- 该方法利用RNA结合蛋白来捕获和映射完整细胞内的RNA-RNA相互作用.
- 深度测序分析结合的RNA片段以重建相互作用网络.
主要成果:
- 已知RNA的二次和三级结构和相互作用被RIC- seq成功地复制.
- 这项技术可以生成人类细胞中的RNA的全球3D交互图.
- 确定了新的非编码RNA目标,RNA拓域和跨作用RNA枢纽.
- 增强剂和促进剂之间的功能连接与对 RNA 相互作用有关.
- 超增强 RNA CCAT1-5L 已被证明与 hnRNPK 和 MYC 调节 RNA 相互作用,通过染色体循环增强 MYC 转录.
结论:
- RIC-seq是全球RNA结构和现场相互作用的强大工具.
- 这项研究揭示了基于RNA的基因表达和基因组组织的新见解.
- 这些发现突显了3DRNA结构在细胞功能和疾病中的重要性.
更多相关视频
12:54Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
06:58Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
Published on: October 7, 2021
相关概念视频
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...