在阿拉比多普西斯 (Arabidopsis) 中,strigolactone信号的转录调节
Lei Wang1,2, Bing Wang3, Hong Yu1
1State Key Laboratory of Plant Genomics, and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
Nature
|June 13, 2020
概括
通过控制基因转录来调节植物的发育. 作为转录因子,SMXL6蛋白抑制基因以保持激素信号平衡.
科学领域:
- 植物生物学
- 分子生物学
- 激素信号传递
背景情况:
- 黄素是植物激素,对植物的发育和相互作用至关重要.
- 像DWARF14 (D14) 和MORE AXILLARY GROWTH 2 (MAX2) 这样的关键蛋白质参与着strigolactone的信号传递.
- 通过strigolactones下游的转录调节仍然在很大程度上没有特征.
研究的目的:
- 在Arabidopsis thaliana中识别受力于strigolactone的基因
- 阐明底层的转录机制的植物发展strigolactone介导.
- 调查SMXL蛋白在strigolactone信号传递中的作用.
主要方法:
- 通过转录基因分析,利用合成的激素来识别反应基因.
- 研究了发芽分支的基因调节,叶子形状和素积累.
- 进行了染色体免疫沉和促进剂结合试验,以研究SMXL6的功能.
主要成果:
- 在Arabidopsis中发现了401个对杆菌素有反应的基因.
- 证明了STRIGOLACTONE对分支1的调节,TCP域蛋白1和ANTHOCYANIN色素1的产生基因.
- 显示SMXL6直接抑制SMXL6,SMXL7和SMXL8的转录,作为一种自我调节的转录因子.
结论:
- 主要激活下游转录以控制植物的发展.
- SMXL6 作为一种 DNA 结合转录因子,可以自主调节strigolactone 信号传递.
- 在植物中通过直接的转录抑制揭示了激素信号调节的新机制.
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