广泛的结构变异对番茄的基因表达和作物改善的主要影响
Michael Alonge1, Xingang Wang2, Matthias Benoit3
1Department of Computer Science, Johns Hopkins University, Baltimore, MD 21218, USA.
Cell
|June 20, 2020
概括
结构变异 (SV) 是作物特征的关键. 这项研究使用番茄的长读序列识别数千种SV,揭示了它们对基因表达和重要的农业特征的广泛影响.
科学领域:
- 基因组学
- 植物生物学
- 农作物科学
背景情况:
- 结构变异对于作物化和改良至关重要.
- 在植物遗传学中,描述 SV 的全谱和影响仍然是一个重大挑战.
研究的目的:
- 在多种多样的番茄品种中全面识别和描述结构变异 (SV).
- 研究 SVs 在基因调节,定量特征变异和作物改进中的作用.
主要方法:
- 长读纳米孔测序100个不同的番茄线.
- 全基因组分析包含14个新的参考组件.
- 量化遗传学和基因组编辑方法.
主要成果:
- 在番茄板上发现了238,490个结构变体.
- 识别了许多影响基因和 cis 调节区域的 SV,导致基因表达的改变.
- 证明 SVs 改变了水果的关键特征 (味道,大小,产量) 和化特征 (收获).
结论:
- 结构变异 (SV) 在基因型与表型关系中起着重要且经常被低估的作用.
- 农产品品种是了解和推动作物改进和育种策略的重要资源.
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