生命王国的蛋白质格局
Johannes B Müller1, Philipp E Geyer1,2, Ana R Colaço3
1Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
Nature
|June 20, 2020
概括
这项研究对100种不同的物种进行了大规模的蛋白质组分析,
科学领域:
- 蛋白质组学
- 比较基因组学
- 进化生物学
背景情况:
- 由于技术的局限,大规模的蛋白质研究 (蛋白质组学) 历来落后于基因组学.
- 之前的研究主要集中在人类蛋白质组上,对跨物种的比较有限.
- 基于质谱的蛋白质组学的进步使得蛋白质的识别和量化更加全面.
研究的目的:
- 对100种分类学上不同的生物进行深入,大规模的蛋白质学研究.
- 建立一个标准化的蛋白质组工作流程,用于跨物种的比较.
- 为探索整个进化范围的功能组织提供一个全面的数据集.
主要方法:
- 使用先进的蛋白质组学工作流程,采用微结构,高度可复制的色谱系统进行分离.
- 在100种不同的物种进行了标准化,深入的蛋白质组分析.
- 应用基于序列的机器学习以实验证实预测的性质.
主要成果:
- 实现了超过200万个和34万个严格的蛋白质识别,有效地将蛋白质的数量与实验证据加倍.
- 证明了普遍的生物策略,高蛋白质分数用于所有王国的蛋白质稳态/折叠和能量生产.
- 在生物体中发现了蛋白质和蛋白质组的显著多样性,新的在线资源促进了功能性比较.
结论:
- 这项研究提供了整个进化谱的生物功能组织的比较观点.
- 蛋白质平衡和能量供应是所有生命形式的基本生物挑战.
- 通过www.proteomesoflife.org可获得的蛋白质组数据强调了普遍的生物原则和显著的物种间多样性.
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