穿孔素与补充剂 (C9) 的第九成分 (C9) 的同质性
Y Shinkai1, K Takio, K Okumura
1Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Nature
|August 11, 1988
概括
研究人员确定了穿孔素的基因,穿孔素是细胞介导细胞解中的关键蛋白质. 这种特定于杀手细胞的蛋白质在进化过程中与补充成分9 (C9) 有关,为其在免疫反应中的作用提供了分子证据.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 珀福林是一种在细胞毒性T淋巴细胞和自然杀手细胞中发现的关键细胞分解因子.
- 了解穿孔素的分子基础对于阐明细胞介导的细胞毒性机制至关重要.
研究的目的:
- 为了确定小鼠自然杀手细胞孔的N端氨基酸序列.
- 为了识别编码 perforin 的互补DNA (cDNA).
- 调查穿孔素和补充成分9 (C9) 之间的结构关系.
主要方法:
- 从小鼠自然杀手细胞系中净化素.
- 净化 perforin 的 N-终端氨基酸测序.
- 基于氨基酸序列的橄核酸探针设计.
- 对细胞毒性T淋巴细胞克隆cDNA库进行查,以识别穿孔素cDNA.
主要成果:
- 确定了穿孔素的N端氨基酸序列.
- 一个编码 perforin 的 cDNA 成功被识别出来.
- 穿孔素的推断初级结构显示在保存区域中与C9相同.
- 证实氨酸表达是特定于杀手细胞系的.
结论:
- 直接的分子证据证实,穿孔素是一种杀手细胞特异性蛋白质.
- 珀福林在进化上与C9相关,这表明它有共同的祖先.
- 这一发现为穿孔素在细胞中介细胞分解中的作用提供了分子基础.
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