在抗原加工和呈现的I类途径中引入可溶性蛋白质
M W Moore1, F R Carbone, M J Bevan
1Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Cell
|September 9, 1988
概括
细胞毒性T淋巴细胞 (CTL) 识别由主要基因相容性复合体 (MHC) I 类分子呈现的. 引入卵胺 (OVA) 直接进入细胞细胞质中,但不是细胞外,对CTL溶解敏感细胞,揭示细胞内处理途径.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 第I类主要组织相容性复合体 (MHC) 糖蛋白向细胞毒性T淋巴细胞 (CTLs) 呈现细胞内.
- 了解与MHC I类联的细胞内机制对于适应性免疫和疫苗开发至关重要.
研究的目的:
- 研究类与I类MHC分子结合的细胞内通路.
- 确定抗原的处理和呈现如何影响CTL识别.
主要方法:
- 在C57BL/6小鼠中生成特定于OVA258-276的H-2Kb受限CTLs.
- 在体外测试中使用合成OVA,CNBr片段和原生OVA来使细胞对CTL介导的溶解敏感.
- 通过pinosomes的透性溶解,OVA的细胞内输送.
主要成果:
- 合成OVA (258-276) 和OVA片段 (242-285,242-273) 通过特定的CTLs使H-2b细胞对溶解敏感.
- 在24小时内与原生OVA进行化,并没有使细胞对识别产生敏感性.
- 对OVA的直接细胞质输送导致了H-2Kb受限决定物的形成,使细胞对溶解敏感.
结论:
- 抗原的细胞内处理和呈现对于由CTLs识别的MHC I类复合物的形成至关重要.
- 抗原进入的途径显著影响其处理和随后在MHC I类分子上的呈现.
- 细胞质递送绕过了细胞外处理障碍,使OVA258-276表位的高效呈现成为可能.
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