在尿路感染中,人体尿模蛋白丝的结构和功能
Gregor L Weiss1, Jessica J Stanisich1, Maximilian M Sauer1
1Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, CH-8093 Zürich, Switzerland.
概括
尿蛋白形成尿管,捕获尿病原体,防止感染. 这项研究揭示了丝结构以及它如何聚集细菌以进行清除.
科学领域:
- 生物化学
- 结构生物学
- 微生物学
背景情况:
- 尿素是人类尿液中最丰富的蛋白质.
- 已知乌罗莫杜林纤维对尿病原体有保护作用,但其结构和保护机制尚不清楚.
研究的目的:
- 阐明尿模蛋白纤维的结构基础.
- 了解乌罗莫杜林对尿道感染的保护机制.
主要方法:
- 使用冷电子断层扫描来确定丝结构.
- 通过N- glycosylation测绘和生物物理测定,确定了uromodulin与细菌粘附素的相互作用.
- 在体外和患者尿液成像中可视化了泌尿模块素与病原体的相互作用.
主要成果:
- 尿模蛋白纤维具有曲的脊柱和突出的手臂.
- 乌罗莫杜林作为1型粘素的多价联体.
- 纤维聚合尿病原体,防止粘附并促进清除.
结论:
- 这项研究揭示了uromodulin对尿路病原体的保护功能的结构基础.
- 这为了解尿道感染和其他生理过程中的尿素作用提供了框架.
相关概念视频
Ureters
1.2K
The ureters are retroperitoneal tubes located on either side of the vertebral column. They are responsible for transporting urine from each kidney to the urinary bladder. These tubes have thick walls and are approximately 25-30 cm long. Their diameter is around 10 mm at the renal pelvis, gradually narrowing to 1 mm as the ureter obliquely enters the posterior bladder wall through the ureteric orifices. The shape of these orifices is slit-like, which helps to prevent urine backflow toward the...
1.2K
Urinary Tract Infection II: Pathophysiology
453
The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
453
Anatomy of the Genitourinary System II: Bladder and Urethra
888
The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...
888
Urinary Tract Infection I: Introduction
422
Urinary tract infections (UTIs) impact various parts of the urinary system, including the kidneys, ureters, bladder, and urethra. These infections are generally bacterial, with Escherichia coli being the most common causative agent, often originating from the gastrointestinal tract. However, other bacteria, such as Staphylococcus saprophyticus, Klebsiella pneumoniae, and Proteus mirabilis, are also known to cause UTIs. The type, location, and underlying complexity of the UTI guide both...
422
Anatomy of the Genitourinary System I: Kidneys and Ureters
358
The upper urinary system comprises two kidneys and two ureters, which are crucial in filtering blood and forming urine.KidneysLocation and Structure:The kidneys are two bean-shaped organs positioned behind the peritoneum on either side of the spine.Kidneys are between the 12th thoracic (T12) and the 3rd lumbar (L3) vertebrae.The position of the liver causes the right kidney to sit slightly lower than the left.Protective Layers:Each kidney is enveloped in a tough, fibrous membrane called the...
358
Fimbriae, Pili, and Axial Filaments
1.2K
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
1.2K


