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通过BRICseq将整个大脑的区域间连接连接到单个动物的神经活动和基因表达

Longwen Huang¹, Justus M Kebschull², Daniel Fürth¹

¹Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

|July 4, 2020

概括

一种名为BRICseq (全脑个体动物连接体测序) 的新方法可以快速,低成本地绘制单个动物的大脑连接. 这种技术将基因表达和大脑活动与神经元连接模式联系起来.

科学领域:

神经科学
基因组学
系统生物学

背景情况:

了解复杂的神经网络需要对连接,活动和基因表达进行大脑范围的测量.
目前的大脑活动和转录组的高通量方法缺乏可比的速度和成本效益,用于绘制区域间的连接,通常需要数百个大脑进行平均化.

研究的目的:

引入BRICseq (全脑个体动物连接组测序),这是绘制大脑连接的新方法.
证明BRICseq在将基因表达和活性与个体动物的神经元连接性相关的实用性.
能够对影响神经元连接的因素进行大规模的比较研究.

主要方法:

BRICseq使用DNA条码和测序进行高通量,个体动物连接组映射.
该方法应用于小鼠新皮质和一个缺乏体的突变BTBR小鼠模型.

主要成果:

在几周内,BRICseq成功地在小鼠中绘制了区域间的连接.
发现空间基因表达模式可以预测区域间的连接性,从而预测活动相关性.
该研究对BTBR小鼠模型中的已知连接病例进行了回顾,验证了BRICseq的准确性.

结论:

BRICseq提供了一个可扩展和具有成本效益的解决方案,用于绘制个体大脑连接组.
这些发现建立了转录组,连接性和活动之间的直接联系,简化了大脑功能的解释.
通过BRICseq,研究人员可以研究各种因素 (年龄,性别,遗传,环境,物种) 如何影响神经元连接,并将其与功能数据整合起来.

关键词:

布里克斯国家其他国家连接组高通量测序中尺度

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