一个T细胞克隆表达两个T细胞受体α基因,但使用一个αβ异构体用于全识别和自我MHC受限抗原识别
M Malissen1, J Trucy, F Letourneur
1Centre d'Immunologie INSERM-CNRS de Marseilles Luminy, France.
Cell
|October 7, 1988
概括
双T细胞的识别来自单个T细胞受体 (TCR) 与两个α链基因. 这种TCR与异性 (-MHC) 和自身MHC交叉反应,挑战了目前T细胞受体基排斥的模型.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- T细胞生物学T细胞生物学
背景情况:
- T细胞受体 (TCRs) 通过识别抗原来调解适应性免疫.
- TCRs通常由α和β链组成,每个链由重新排列的基因段编码.
- 基排斥通常只能确保每个T细胞只有一个TCR特异性.
研究的目的:
- 描述T细胞受体α和β链在双反应性T细胞克隆中的重新排列.
- 调查T细胞双重特异性的遗传基础.
- 探索对T细胞受体基排除的影响.
主要方法:
- 描述T细胞受体α和β链基因重组的特征.
- 对T细胞受体基因mRNA转录的分析.
- 基因转移实验使用转染的α和β基因.
- 评估T细胞对异性MHC和自我MHC受限抗原的识别.
主要成果:
- 该T细胞克隆对其β链基因表现出基排斥,只有一次生产性重新排列.
- 意想不到的是,克隆显示了两个有效的Vα基因重排,两者都转录为mRNA.
- 基因转移实验表明,单个α-β组合足以进行双重识别,表明交叉反应性.
结论:
- 双T细胞识别可以来自单个具有交叉反应特异性的αβT细胞受体.
- 这一发现表明,单个TCR可以识别alo-MHC和自我-MHC.
- 两个生产性阿尔法链基因的存在引发了关于T细胞中等位列排斥的调节的问题.
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