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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Updated: Dec 14, 2025

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
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Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

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一种生物直角小分子选择性聚合物"点击酶"

Junfeng Chen1, Ke Li1, Sarah E Bonson1

  • 1Department of Chemistry, University of Illinois, Urbana, Illinois 61801, United States.

Journal of the American Chemical Society
|July 16, 2020
PubMed
概括

研究人员开发了一种新型单链纳米粒子 (SCNP) 催化剂,可选择性地向小分子进行"点击"反应. 这种人造克利克酶使细胞外生物对角化学成为可能,有助于选药物配体,并在没有细胞干扰的情况下合成抗癌剂.

科学领域:

  • 聚合物化学
  • 催化剂
  • 纳米技术

背景情况:

  • 合成聚合物支架可以控制分子相互作用.
  • 铜催化酸环添加 (CuAAC) 是一个关键的生物对角反应.
  • 开发选择性催化剂对于精确的化学操作至关重要.

研究的目的:

  • 设计具有选择性小分子激活门特性的单链纳米粒子 (SCNP) 催化剂.
  • 为了研究催化剂在细胞外的化学作用.
  • 为了证明在配体查和现场药物合成中的应用.

主要方法:

  • 含铜的SCNP与聚乙烯糖醇 (PEG) 组的合成.
  • 将SCNP催化剂的活性与含有基的同类催化剂进行比较.
  • 评估SCNP催化剂的细胞透性和细胞外活动.
  • 用SCNP催化选蛋白质结合体和PROTAC类分子的演示.
  • 在细胞外抗癌药物合成和查中使用SCNP.

主要成果:

  • 采用PEG移植的SCNP催化剂选择性地与小分子反应,不同于与蛋白质和小分子反应的氨功能化催化剂.
  • 该SCNP催化剂表现出对细胞吸收的抗性,使细胞外催化成为可能.

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  • 验证原则的应用成功地表明了SCNP在配体查和细胞外药物合成中的实用性.
  • 这种SCNP催化剂促进了局部抗癌药物合成和细胞外查.

    • 结论:

    • 在SCNPs中接可以为小分子生物对角反应提供选择性.
    • 开发的SCNP催化剂可实现受控的细胞外点击化学反应.
    • 这种SCNP催化剂为药物发现和开发中的应用提供了多功能平台,包括连接体查和向药物合成.