通过氧化产生细菌化学石质自
Hang Yu1, Jared R Leadbetter2,3
1Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, USA.
Nature
|July 17, 2020
概括
研究人员发现了利用氧化成长的微生物,完成了关键的生物地球化学循环. 这一发现扩大了我们对微生物能量代谢和地球能量代谢的理解.
科学领域:
- 微生物学 微生物学
- 地质化学 地质化学
- 生物地质化学生物地质化学
背景情况:
- 氧化理论上支持微生物生长,但缺乏实验证明.
- 化学自营微生物利用无机化合物获得能量和固定碳.
研究的目的:
- 为了证明和描述由 (Mn(II)) 氧化推动的微生物生长.
- 识别所涉及的微生物及其代谢途径.
主要方法:
- 精制丰富培养物以分离一个Mn{\displaystyle Mn{\text{II}}}}-氧化共培.
- 使用13CO2进行稳定同位素探测,以追踪碳固定.
- 转录组分析以阐明代谢途径.
主要成果:
- 两个细菌的共同培养",Candidatus Manganitrophus noduliformans"和Ramlibacter lithotrophicus,证明了依赖于Mn2氧化的指数级增长.
- Mn(II) 氧化产生与细菌细胞相关的氧化结节.
- 稳定同位素探测证实了与Mn2氧化相关的13CO2固定在生物质中.
结论:
- 这项研究提供了第一个通过氧化推动微生物生长的实验证据.
- 这些发现扩大了已知的无机代谢的多样性,并完成了的生物地球化学循环.
- 已识别的微生物通路将细胞外氧化与有氧能量保存和自二氧化碳固定联系起来.
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