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内生干细胞的心脏再生和心肌细胞的增殖:争议,谬论和进展
Lingjuan He1, Ngoc B Nguyen2,3, Reza Ardehali2,3
1State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China (L.H., B.Z.).
Circulation
|July 21, 2020
概括
心脏干细胞 (CSC) 曾被认为能再生心脏组织,但最近的研究质疑它们的存在和肌性潜力. 现在的研究重点是心肌细胞的增殖和改进的细胞追踪方法,
科学领域:
- 心血管生物学
- 复原医学
- 干细胞研究
背景情况:
- 缺血性心脏病和心肌梗塞导致心肌细胞损失,不良重塑和心力衰竭,推动寻找心脏再生策略.
- 心脏干细胞 (CSC) 已被提议作为再生受损心肌的治疗来源,其中一些显示出临床前景.
- 最近的细胞追踪研究对之前确定的CSC的基本生物学和肌性潜力产生了怀疑.
研究的目的:
- 审查有关心脏干细胞 (CSC) 在心脏再生方面的历史发展和最近的挑战.
- 评估心肌细胞增殖作为心肌修复机制的新兴作用.
- 批判性地评估研究CSC和心肌细胞增殖的方法优势和局限性.
主要方法:
- 在过去十年中对心脏干细胞 (CSC) 的研究进行了全面的文献综述.
- 对最近的细胞追踪研究进行分析,质疑CSC的起源和功能.
- 评估用于CSC和心肌细胞增殖研究的方法,包括细胞标记和血统追踪技术.
主要成果:
- 细胞追踪研究的证据引发了有关先前发现的CSC存在和再生能力的重大担忧.
- 预先存在的心肌细胞的增殖正在成为心肌细胞修复的潜在贡献者.
- 过去和当前研究的方法限制需要改进的技术来准确评估心肌细胞的生成和再生.
结论:
- 心脏再生领域需要重新评估心脏干细胞 (CSC) 的作用和存在.
- 未来的研究应该集中在单细胞标记和谱系追踪技术的进步上,以准确了解心肌细胞的发育潜力和命运.
- 拥抱技术进步对于克服不准确性和提高对心脏再生的理解至关重要.
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