在Pseudomonas aeruginosa生物膜中促进有效的细胞外电子转移
Scott H Saunders1, Edmund C M Tse2, Matthew D Yates3
1Division of Biology and Biological Engineering, Caltech, Pasadena, CA, USA.
Cell
|August 9, 2020
概括
素通过穿电子来促进细菌生物膜代谢. 细胞外DNA (eDNA) 结合这些分子,使得电子能有效转移,并防止生物膜的损失.
科学领域:
- 微生物学
- 生物化学
- 环境科学
背景情况:
- 细菌生物膜含有有限的基本营养素和电子受体/捐赠体的子群.
- 细胞外电子穿对于这些亚群体的代谢活动至关重要.
- 一个关键的挑战是了解这些穿器如何留在生物膜中以促进电子传输.
研究的目的:
- 研究酶在Pseudomonas aeruginosa生物膜中调解细胞外电子转移 (EET) 的机制.
- 确定细胞外DNA (eDNA) 在保留phenazines和促进ET中的作用.
主要方法:
- 在体外实验中评估具有和没有DNA的酶之间的电子交换.
- 在生物膜中检查电子转移的体内研究.
- 对eDNA结合的分析.
主要成果:
- 氨酸,特别是氨酸 (PYO) 和氨酸胺,与eDNA结合,增加它们在生物膜基质中的保留.
- eDNA促进了酶之间的直接氧化还原反应和电子交换.
- 生物膜eDNA支持氧化还原活性介质之间的快速电子转移.
- PYO:eDNA相互作用建立了一个高效的氧化还原循环,EET的速率超过了生物膜中的酶损失.
结论:
- 细胞外DNA对于保持细菌生物膜中的酶至关重要.
- 氨酸:eDNA相互作用对于生物膜中有效的细胞外电子转移 (EET) 是至关重要的.
- 这种机制通过启用氧化还原循环来支持细菌在生物膜中的代谢活动.
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