相关实验视频
Updated: Dec 12, 2025
14:16
Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
22.7K
光滴滴细胞测量用于细胞表型追踪
Xiaolong Yang1, Wenhan Liu2, Darren Chi-Hang Chan1
1Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S3M2, Canada.
Journal of the American Chemical Society
|August 14, 2020
概括
这项研究引入光滴状细胞计 (FDC) 用于活癌细胞单细胞分析. 这种方法甚至可以对非常小的细胞集合进行详细的表型分析.
科学领域:
- 生物技术
- 癌症研究
- 单细胞分析
背景情况:
- 鉴定异质活癌细胞表型对于了解癌症至关重要.
- 现有的单细胞分析方法面临着低丰度细胞群的挑战.
- 很难准确地描述小细胞集合.
研究的目的:
- 开发一种低丰度活癌细胞单细胞分析的方法.
- 为了从小样本中进行癌细胞的表型分析.
- 推进癌症研究中的单细胞分析能力.
主要方法:
- 使用滴状微流体平台开发光滴状细胞计量 (FDC).
- 采用生物标志物特定的酶体光试验.
- 使用DNA功能化的抗体检测细胞目标.
主要成果:
- 实现活癌细胞表型分析的单细胞分辨率.
- 在活癌细胞上成功鉴定了多种表面标记物.
- 对小细胞采集 (<40个细胞) 的有效分析.
结论:
- 光滴状细胞测量 (FDC) 能够对低丰度细胞进行强大的单细胞分析.
- 这种方法可以根据表面标记进行详细的活细胞表型分析.
- FDC为癌症研究提供了有价值的工具,特别是在有限的细胞样本中.
相关概念视频
01:23Flow Cytometry
15.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
In...
15.2K
01:19Protein Dynamics in Living Cells
2.5K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.5K