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针对COVID-19的热稳定mRNA疫苗

Na-Na Zhang¹, Xiao-Feng Li², Yong-Qiang Deng²

¹State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China; School of Medicine, Tsinghua University, Beijing 100084, China.

|August 16, 2020

概括

一种新型的脂质纳米粒子封装信使RNA (mRNA) 疫苗ARCoV针对SARS-CoV-2受体结合域,在临床前模型中显示出强大的中和抗体和完整的保护. 这种COVID-19候选疫苗具有室温稳定性.

科学领域:

疫苗学
分子生物学
免疫学

背景情况:

由于COVID-19的流行,需要快速研发疫苗.
传递 RNA (mRNA) 疫苗为新出现的传染病提供了多功能平台.
脂质纳米粒子 (LNP) 封装增强了mRNA疫苗的输送和稳定性.

研究的目的:

开发和评估一种用于预防COVID-19的新型mRNA疫苗候选人ARCoV.
在临床前模型中评估ARCoV的免疫性和保护功效.
确定ARCoV疫苗的储存稳定性和配方特性.

主要方法:

开发一种脂纳米粒子封装的mRNA疫苗,编码SARS-CoV-2受体结合域 (RBD).
用ARCoV mRNA-LNP疫苗对小鼠和非人类灵长类动物进行肌肉免疫.
对中和抗体标位和Th1偏差细胞反应的评估.
挑战使用适应小鼠SARS-CoV-2菌株的研究来评估疫苗的有效性.
在液体配方中评估ARCoV在室温中的稳定性.

主要成果:

在ARCoV mRNA-LNP免疫接种过程中产生了针对SARS-CoV-2的强有力的中和抗体.
在免疫小鼠和非人类灵长类动物中观察到Th1偏差的细胞免疫反应.
两剂ARCoV在小鼠中提供了对SARS-CoV-2挑战的完整保护.
抗冠状病毒疫苗是液体的,在室温下保持稳定至少一周.

结论:

这种ARCoV mRNA疫苗候选产生的免疫力强烈,可以对抗SARS-CoV-2.
在临床前模型中,ARCoV显示出完全的保护性,突出显示了其作为COVID-19疫苗的潜力.
由于ARCoV的室温稳定性,为疫苗分发提供了后勤优势.
目前,ARCoV 正在进入第一阶段临床试验,以进一步评估其对人类的安全性和有效性.

关键词:

美国冠状病毒脂质纳米粒子 mRNA疫苗的使用情况适应小鼠的菌株非人类灵长类动物进行保护

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