严重的COVID-19是由失调的骨髓细胞区分标记的
Jonas Schulte-Schrepping1, Nico Reusch1, Daniela Paclik2
1Life and Medical Sciences (LIMES) Institute, University of Bonn, Germany.
Cell
|August 19, 2020
概括
严重的COVID-19涉及紧急骨髓形成和功能失调的中性粒细胞,与炎症单细胞的轻微病例不同. 这项研究详细介绍了SARS-CoV-2感染的免疫细胞变化.
科学领域:
- 免疫学
- 病毒学
- 血液学
背景情况:
- 2019年新冠病毒病 (COVID-19) 可能从轻微的呼吸系统疾病到严重的呼吸系统衰竭.
- 在轻微的COVID-19防护和严重的疾病发病的基础上的免疫机制,特别是关于中性粒细胞和免疫失调的免疫机制,尚未完全理解.
研究的目的:
- 研究轻度和严重的COVID-19免疫细胞组成和激活的动态变化.
- 在单细胞水平上阐明对SARS-CoV-2感染的系统性免疫反应.
主要方法:
- 在全血和外围血单核细胞上利用单细胞RNA测序和单细胞蛋白质组学.
- 在两个群体和两个中心分析了109个人的242个样本.
主要成果:
- 轻微的COVID-19显示HLA-DRhiCD11chi炎症单细胞增加,具有干扰素刺激的基因特征.
- 严重的COVID-19的特征是中性粒细胞前体 (紧急骨髓形成),功能障碍的成熟中性粒细胞和HLA-DRlo单细胞.
结论:
- 这项研究揭示了严重的COVID-19中骨髓细胞区的深刻变化.
- 对SARS-CoV-2感染的系统性免疫反应的详细见解,突出了轻度与严重疾病的不同骨髓细胞特征.
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